Part:BBa_K4299002

Feel the uric acid signal, activate the uric acid oxidase

Added by Sesame_Shenzhen

3 Determining the collective efficacy of uric acid sensitive oxidation effect.

Figure 1

We introduced both systems, the Uric acid promoter and the catabolic enzyme, into E.coli nissle 1917, and simulated the conditions inside the gut. conditions were:

•pH of 7

•The temperature of 37 degrees Celsius

•Hypoxia (hypoxic bag used)

•Add bile salts

•Pancreatic juice

•Liquid LB medium

•Adjust the uric acid concentration to 2000 μM. Experimental Process:

Co-culture the bacteria with the above materials.

Co-incubate for 48 hours, incubate at 37°C on a shaker at 180 rpm

Test for uric acid concentration

Figure 2

The final degradation rate of the control group was about 10%, and the experimental group was about 45%, proving the functionality of our system. In conclusion, we believe our system can be functional under the conditions of the intestine.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 546
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1014
Illegal AgeI site found at 1365
Illegal AgeI site found at 1836
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 282
Illegal SapI.rc site found at 246